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02886nam a22003015i 4500 |
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978-1-59259-259-3 |
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20191027051737.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592592593
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| 024 |
7 |
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|a 10.1385/1592592597
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a DNA Topoisomerase Protocols
|b Volume I: DNA Topology and Enzymes /
|c edited by Mary-Ann Bjornsti, Neil Osheroff.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XV, 327 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 94
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| 505 |
0 |
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|a to DNA Topoisomerases -- Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis -- Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers -- Analysis of Altered DNA Structures -- Purification of Supercoiled Plasmid DNA -- Purification and Use of DNA Minicircles with Different Linking Numbers -- Isolation of Kinetoplast DNA -- Isolation of Knotted DNA from Coliphage P4 -- Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis -- Sedimentation Analysis of Bacterial Nucleoid Structure -- Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy -- Methods for Analyzing DNA Bending -- Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination -- Overexpression and Purification of Bacterial DNA Gyrase -- Overexpression and Purification of Bacterial DNA Topoisomerase I -- Overexpression and Purification of Escherichia coliDNA Topoisomerase III -- Overexpression and Purification of Bacterial Topoisomerase IV -- Purification of the Bacteriophage T4 Type II DNA Topoisomerase -- Overexpression and Purification of DNA Topoisomerase I from Yeast -- Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast -- Purification of DNA Topoisomerase II from Drosophila melanogaster -- Purification of DNA Topoisomerase I from Human Placenta -- Purification of Baculovirus-Expressed Human DNA Topoisomerase I -- Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography -- Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II -- Immunoblot Analysis and Band Depletion Assays -- Visualization of DNA Topoisomerases by Electron Microscopy -- Appendix: Compendium of DNA Topoisomerase Sequences.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Bjornsti, Mary-Ann.
|e editor.
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| 700 |
1 |
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|a Osheroff, Neil.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592592597
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