DNA Topoisomerase Protocols Volume I: DNA Topology and Enzymes /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edition: | 1st ed. 1999. |
| Series: | Methods in Molecular Biology,
94 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592597 |
Table of Contents:
- to DNA Topoisomerases
- Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis
- Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers
- Analysis of Altered DNA Structures
- Purification of Supercoiled Plasmid DNA
- Purification and Use of DNA Minicircles with Different Linking Numbers
- Isolation of Kinetoplast DNA
- Isolation of Knotted DNA from Coliphage P4
- Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis
- Sedimentation Analysis of Bacterial Nucleoid Structure
- Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy
- Methods for Analyzing DNA Bending
- Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination
- Overexpression and Purification of Bacterial DNA Gyrase
- Overexpression and Purification of Bacterial DNA Topoisomerase I
- Overexpression and Purification of Escherichia coliDNA Topoisomerase III
- Overexpression and Purification of Bacterial Topoisomerase IV
- Purification of the Bacteriophage T4 Type II DNA Topoisomerase
- Overexpression and Purification of DNA Topoisomerase I from Yeast
- Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast
- Purification of DNA Topoisomerase II from Drosophila melanogaster
- Purification of DNA Topoisomerase I from Human Placenta
- Purification of Baculovirus-Expressed Human DNA Topoisomerase I
- Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography
- Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II
- Immunoblot Analysis and Band Depletion Assays
- Visualization of DNA Topoisomerases by Electron Microscopy
- Appendix: Compendium of DNA Topoisomerase Sequences.