Quantitative PCR Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kochanowski, Bernd. (Editor ), Reischl, Udo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edición:1st ed. 1999.
Colección:Methods in Molecular Medicine, 26
Materias:
Acceso en línea:https://doi.org/10.1385/0896035182
Tabla de Contenidos:
  • Reviews
  • Quantitative PCR
  • General Principles of Quantitative PCR
  • Effects of Collection, Processing, and Storage on RNA Detection and Quantification
  • Quantitative RT-PCR
  • Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
  • Comparison of Competitive PCR and Positive Control-Based PCR
  • Protocols
  • End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA
  • Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence
  • Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid Chromatography (HPLC)
  • Quantifying Amplicons with ELISA
  • Competitive PCR Quantitation Utilizing a Microtiter Plate Based Format for the Detection of PCR Products
  • Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Antisense Competitors
  • Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA)
  • Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene
  • Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing
  • High Resolution PCR Quantitation by AmpliSensor Assay
  • Construction of Polycompetitors for Competitive PCR
  • Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
  • A Stochastic PCR Approach for RNA Quantification in Multiple Samples
  • Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes.