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02665nam a22002895i 4500 |
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978-1-59259-264-7 |
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20190618051838.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
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|a 9781592592647
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7 |
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|a 10.1385/1592592643
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Lipidation Protocols
|c edited by Michael H. Gelb.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a X, 240 p. 35 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 116
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|a In Vitro Analysis of GPI Biosynthesis in Mammalian Cells -- Selection of Mammalian Cell Mutants in GPI Biosynthesis -- Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins -- Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins -- Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein ?-, ?-, and ?-Subunits -- Analysis of Lipids in Caveolae -- Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling -- Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae -- Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol -- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins -- Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits -- Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes -- Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides -- Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides -- Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase -- Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells -- Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells.
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| 650 |
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|a Biochemistry.
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| 650 |
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4 |
|a Biochemistry, general.
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| 700 |
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|a Gelb, Michael H.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592643
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