Protein Lipidation Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edición: | 1st ed. 1999. |
| Colección: | Methods in Molecular Biology,
116 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592592643 |
Tabla de Contenidos:
- In Vitro Analysis of GPI Biosynthesis in Mammalian Cells
- Selection of Mammalian Cell Mutants in GPI Biosynthesis
- Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins
- Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins
- Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein ?-, ?-, and ?-Subunits
- Analysis of Lipids in Caveolae
- Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling
- Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae
- Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol
- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins
- Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits
- Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes
- Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides
- Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides
- Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase
- Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells
- Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells.