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02791nam a22003015i 4500 |
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978-1-59259-269-2 |
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20190618051800.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592592692
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| 024 |
7 |
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|a 10.1385/0896035255
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Mycoplasma Protocols
|c edited by Roger J. Miles, Robin A. J. Nicholas.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XIII, 330 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 104
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| 505 |
0 |
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|a Medical Significance of Mycoplasmas -- The Veterinary Significance of Mycoplasmas -- Recovery of Human Mycoplasmas -- Recovery of Mycoplasmas from Animals -- Recovery of Mycoplasmas from Birds -- Cultivation of Ureaplasmas -- Quality Control Testing of Mycoplasma Medium -- Biochemical Characteristics in Mycoplasma Identification -- Enzyme Analysis -- Determination of Substrate Utilization Rates by Mycoplasmas -- Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests -- Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot) -- Identification of Mycoplasmas by Immunofluorescence -- Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs -- Immunohistochemical Staining of Fixed Tissues -- Extraction of DNA from Mycoplasmas -- Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers -- PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC -- Characterization of Mycoplasmas by RAPD Fingerprinting -- Classification of Isolates by DNA-DNA Hybridization -- Insertion Sequence Analysis -- Detection of Mycoplasmas in Cell Cultures by Cultural Methods -- Detection of Mycoplasmas in Cell Culture by Fluorescence Methods -- Transformation of Mycoplasmas -- Transposon Mutagenesis -- Demonstration of Extrachromosomal Elements -- Expression of Foreign Genes in Acholeplasma laidlawii -- Mycoplasma Gene Expression in Escherichia coli -- Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins -- Immunological and Biochemical Characterization of Membrane Proteins -- Detection and Analysis of Mycoplasma Adhesins -- Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens.
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Miles, Roger J.
|e editor.
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| 700 |
1 |
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|a Nicholas, Robin A. J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/0896035255
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