Mycoplasma Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Miles, Roger J. (Editor ), Nicholas, Robin A. J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edición:1st ed. 1998.
Colección:Methods in Molecular Biology, 104
Materias:
Acceso en línea:https://doi.org/10.1385/0896035255
Tabla de Contenidos:
  • Medical Significance of Mycoplasmas
  • The Veterinary Significance of Mycoplasmas
  • Recovery of Human Mycoplasmas
  • Recovery of Mycoplasmas from Animals
  • Recovery of Mycoplasmas from Birds
  • Cultivation of Ureaplasmas
  • Quality Control Testing of Mycoplasma Medium
  • Biochemical Characteristics in Mycoplasma Identification
  • Enzyme Analysis
  • Determination of Substrate Utilization Rates by Mycoplasmas
  • Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests
  • Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot)
  • Identification of Mycoplasmas by Immunofluorescence
  • Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs
  • Immunohistochemical Staining of Fixed Tissues
  • Extraction of DNA from Mycoplasmas
  • Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers
  • PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC
  • Characterization of Mycoplasmas by RAPD Fingerprinting
  • Classification of Isolates by DNA-DNA Hybridization
  • Insertion Sequence Analysis
  • Detection of Mycoplasmas in Cell Cultures by Cultural Methods
  • Detection of Mycoplasmas in Cell Culture by Fluorescence Methods
  • Transformation of Mycoplasmas
  • Transposon Mutagenesis
  • Demonstration of Extrachromosomal Elements
  • Expression of Foreign Genes in Acholeplasma laidlawii
  • Mycoplasma Gene Expression in Escherichia coli
  • Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins
  • Immunological and Biochemical Characterization of Membrane Proteins
  • Detection and Analysis of Mycoplasma Adhesins
  • Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens.