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03733nam a22003015i 4500 |
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978-1-59259-270-8 |
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20191027061802.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
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|a 9781592592708
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|a 10.1385/1592592708
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Molecular Embryology
|c edited by Paul T. Sharpe, Ivor Mason.
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|a 1st ed. 1999.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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|a XVI, 756 p.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 97
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|a The Mouse Embryo -- The Mouse as a Developmental Model -- Culture of Postimplantation Mouse Embryos -- Organ Culture in the Analysis of Tissue Interactions -- Treatment of Mice with Retinoids In Vivo and In Vitro -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Gene Trapping in Mouse Embryonic Stem Cells -- Production and Growth of Conditionally Immortal Cell Lines from the H-2Kb tsA58 Transgenic Mouse -- Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Mouse Primordial Germ Cells -- Chicken Embryo -- The Avian Embryo -- Chick Embryos -- New Culture -- Grafting Hensen's Node -- Grafting of Somites -- Notochord Grafts -- Transplantation of Avian Neural Tissue -- Grafting of Apical Ridge and Polarizing Region -- Tissue Recombinations in Collagen Gels -- Quail-Chick Chimeras -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Amphibian Embryo -- An Overview of Xenopus Development -- Mesoderm Induction Assays -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- A Method for Generating Transgenic Frog Embryos -- Axolotl/newt -- Zebrafish -- The Zebrafish -- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development -- Transgenic Zebrafish -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function -- Retinoids in Nonmammalian Embryos -- Monvertevrate Chordates -- Protochordates -- Retroviruses -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Differential Display of Eukaryotic mRNA -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Single-Cell RT-PCR cDNA Subtraction -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- In Situ Hybridization to RNA in Whole Embryos -- Wholemount In Situ Hybridization to Xenopus Embryos -- Wholemount In Situ Hybridization to Amphioxus Embryos -- In Situ Hybridization to Sections (Nonradioactive) -- Immunohistochemistry Using Polyester Wax -- Immunohistochemistry on Whole Embryos -- Whole Embryo Assays for Programmed Cell Death -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos -- Protein Techniques -- Microscopy and Photography -- Microscopy and Photomicrography Techniques.
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|a Cell biology.
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650 |
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|a Cell Biology.
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700 |
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|a Sharpe, Paul T.
|e editor.
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700 |
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|a Mason, Ivor.
|e editor.
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710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1385/1592592708
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