Molecular Embryology

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Sharpe, Paul T. (Editor), Mason, Ivor. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edition:1st ed. 1999.
Series:Methods in Molecular Biology, 97
Subjects:
Online Access:https://doi.org/10.1385/1592592708
Table of Contents:
  • The Mouse Embryo
  • The Mouse as a Developmental Model
  • Culture of Postimplantation Mouse Embryos
  • Organ Culture in the Analysis of Tissue Interactions
  • Treatment of Mice with Retinoids In Vivo and In Vitro
  • Cell Grafting and Labeling in Postimplantation Mouse Embryos
  • Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs
  • Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells
  • Gene Trapping in Mouse Embryonic Stem Cells
  • Production and Growth of Conditionally Immortal Cell Lines from the H-2Kb tsA58 Transgenic Mouse
  • Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos
  • Application of lacZ Transgenic Mice to Cell Lineage Studies
  • Mouse Primordial Germ Cells
  • Chicken Embryo
  • The Avian Embryo
  • Chick Embryos
  • New Culture
  • Grafting Hensen's Node
  • Grafting of Somites
  • Notochord Grafts
  • Transplantation of Avian Neural Tissue
  • Grafting of Apical Ridge and Polarizing Region
  • Tissue Recombinations in Collagen Gels
  • Quail-Chick Chimeras
  • Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo
  • Amphibian Embryo
  • An Overview of Xenopus Development
  • Mesoderm Induction Assays
  • Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian
  • A Method for Generating Transgenic Frog Embryos
  • Axolotl/newt
  • Zebrafish
  • The Zebrafish
  • Small-Scale Marker-Based Screening for Mutations in Zebrafish Development
  • Transgenic Zebrafish
  • Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function
  • Retinoids in Nonmammalian Embryos
  • Monvertevrate Chordates
  • Protochordates
  • Retroviruses
  • Gene Transfer to the Rodent Embryo by Retroviral Vectors
  • Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses
  • Molecular Techniques
  • Subtractive Hybridization and Construction of cDNA Libraries
  • Differential Display of Eukaryotic mRNA
  • RT-PCR on Embryos Using Degenerate Oligonucleotide Primers
  • Single-Cell RT-PCR cDNA Subtraction
  • In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections
  • In Situ Hybridization to RNA in Whole Embryos
  • Wholemount In Situ Hybridization to Xenopus Embryos
  • Wholemount In Situ Hybridization to Amphioxus Embryos
  • In Situ Hybridization to Sections (Nonradioactive)
  • Immunohistochemistry Using Polyester Wax
  • Immunohistochemistry on Whole Embryos
  • Whole Embryo Assays for Programmed Cell Death
  • Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos
  • Protein Techniques
  • Microscopy and Photography
  • Microscopy and Photomicrography Techniques.