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04338nam a22002895i 4500 |
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978-1-59259-271-5 |
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20191027152522.0 |
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cr nn 008mamaa |
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100301s2002 xxu| s |||| 0|eng d |
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|a 9781592592715
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| 024 |
7 |
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|a 10.1385/1592592716
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Malaria Methods and Protocols
|c edited by Denise L. Doolan.
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| 250 |
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|a 1st ed. 2002.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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| 300 |
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|a XV, 631 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Medicine,
|v 72
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| 505 |
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|a Assessing Risk of Infection and Severity of Disease -- Vector Incrimination and Entomological Inoculation Rates -- Epidemiological Measures of Risk of Malaria -- Laboratory Models -- Maintenance of the Plasmodium berghei Life Cycle -- Mouse Models for Pre-Erythrocytic-Stage Malaria -- Mouse Models for Erythrocytic-Stage Malaria -- Nonhuman Primate Models -- Nonhuman Primate Models -- Diagnosis and Typing -- Vector Analysis -- Genotyping of Plasmodium spp. -- Genotyping of Plasmodium falciparum -- Microsatellite Analysis in Plasmodium falciparum -- Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR -- Quantitation of Liver-Stage Parasites by Competitive RT-PCR -- Molecular Biology Techniques -- Extraction and Purification of Plasmodium Total RNA -- Extraction and Purification of Plasmodium Parasite DNA -- Southern Blotting of Parasite DNA -- SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins -- Nested PCR Analysis of Plasmodium Parasites -- RFLP Analysis -- Analysis of Gene Expression by RT-PCR -- In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites -- Purification of Chromosomes from Plasmodium falciparum -- Construction of Genomic Libraries from the DNA of Plasmodium Species -- Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA -- Construction of Plasmodium falciparum ? cDNA Libraries -- Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization -- Construction and Screening of YAC Libraries -- Episomal Transformation of Plasmodium berghei -- Gene Targeting in Plasmodium berghei -- Immunological Techniques -- Peptide Vaccination -- DNA Vaccination -- Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses -- Assessing CD4+Helper T-Lymphocyte Responses by Lymphoproliferation -- Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice -- Cell Trafficking -- Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry -- Cytokine Analysis by Intracellular Staining -- Assessment of Antigen-Specific CTL- and CD8+-Dependent IFN-? Responses in Mice -- Assessing Antigen-Specific CD8+ CTL Responses in Humans -- Human Antibody Subclass ELISA -- Systemic Nitric Oxide Production in Human Malaria -- Systemic Nitric Oxide Production in Human Malaria -- Cell Biology Techniques -- In Vitro Culture of Plasmodium Parasites -- Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator -- Hepatic Portal Branch Inoculation -- Hepatocyte Perfusion, Isolation, and Culture -- Inhibition of Sporozoite Invasion -- Inhibition of Liver-Stage Development Assay -- T-Cell Mediated Inhibition of Liver-Stage Development Assay -- Antibody-Dependent Cellular Inhibition Assay -- Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA -- Analysis of CSA-Binding Parasites and Antiadhesion Antibodies -- Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow -- Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays -- Triton X-114 Phase Partitioning for Antigen Characterization -- Immunoprecipitation for Antigen Localization -- Testing Interventions For Malaria Control -- Field Trials.
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| 650 |
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|a Infectious diseases.
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| 650 |
1 |
4 |
|a Infectious Diseases.
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| 700 |
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|a Doolan, Denise L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592716
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