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04069nam a22002895i 4500 |
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978-1-59259-397-2 |
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20190618131647.0 |
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cr nn 008mamaa |
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100301s2003 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592593972
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| 024 |
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|a 10.1385/1592593976
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Kinase C Protocols
|c edited by Alexandra C. Newton.
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| 250 |
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|a 1st ed. 2003.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2003.
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| 300 |
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|a XVI, 584 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 233
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|a The Ins and Outs of Protein Kinase C -- Early Studies of Protein Kinase C -- Expression and Purification -- Expression and Purification of Protein Kinase C from Insect Cells -- Expression and Purification of Protein Kinase C? from Bacteria -- Measuring the Activity of Protein Kinase C -- Complexities in Protein Kinase C Activity Assays -- Enzyme Assays for Protein Kinase C Activity -- Subcellular Translocation of Protein Kinase C -- Measuring the Interaction of Protein Kinase C with Membranes -- Measuring the Interaction of Protein Kinase C with Membranes -- Fluorescence Imaging of Protein Kinase C Translocation in Living Cells -- Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles -- Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C -- [3H]Phorbol 12,13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins -- Measuring the Phosphorylation of Protein Kinase C -- Protein Kinase C Phosphorylation -- Pulse-Chase Analysis of Protein Kinase C -- PDK-1 and Protein Kinase C Phosphorylation -- In Vitro Autophosphorylation of Protein Kinase C Isozymes -- Tyrosine Phosphorylation of Protein Kinase C -- Methods to Study Dephosphorylation of Protein Kinase C In Vivo -- Phosphopeptide-Specific Antibodies to Protein Kinase C -- Identifying Protein Kinase C Substrates -- Identifying Protein Kinase C Substrates -- A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases -- Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries -- Structural Analysis of Protein Kinase C -- Structural Analysis of Protein Kinase C -- Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms -- Crystallization of the Protein Kinase C? C1B Domain -- Methods for Detecting Binding Proteins -- Methods for Detecting Binding Proteins -- Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis -- Glutathione S-Transferase Pull-Down Assay -- Overlay Method for Detecting Protein-Protein Interactions -- An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates -- Functional Proteomic Analysis of the Protein Kinase C Signaling System -- Pharmacological Probes for Protein Kinase C -- Pharmacological Probes for Protein Kinase C -- Applications of Inhibitors for Protein Kinase C and Their Isoforms -- Phorbol Esters as Probes for the Study of Protein Kinase C Function -- Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs -- Genetic Approaches to Studying Protein Kinase C -- Genetic Approaches to Studying Protein Kinase C -- Animal Models in the Study of Protein Kinase C Isozymes -- Genetic Manipulation of Protein Kinase C In Vivo -- Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms -- Protein Kinase C in Disease -- Protein Kinase C in Disease -- Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models -- Alcohol Addiction.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
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|a Newton, Alexandra C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592593976
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