Protein Kinase C Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 1st ed. 2003. |
| Colección: | Methods in Molecular Biology,
233 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592593976 |
Tabla de Contenidos:
- The Ins and Outs of Protein Kinase C
- Early Studies of Protein Kinase C
- Expression and Purification
- Expression and Purification of Protein Kinase C from Insect Cells
- Expression and Purification of Protein Kinase C? from Bacteria
- Measuring the Activity of Protein Kinase C
- Complexities in Protein Kinase C Activity Assays
- Enzyme Assays for Protein Kinase C Activity
- Subcellular Translocation of Protein Kinase C
- Measuring the Interaction of Protein Kinase C with Membranes
- Measuring the Interaction of Protein Kinase C with Membranes
- Fluorescence Imaging of Protein Kinase C Translocation in Living Cells
- Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles
- Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C
- [3H]Phorbol 12,13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins
- Measuring the Phosphorylation of Protein Kinase C
- Protein Kinase C Phosphorylation
- Pulse-Chase Analysis of Protein Kinase C
- PDK-1 and Protein Kinase C Phosphorylation
- In Vitro Autophosphorylation of Protein Kinase C Isozymes
- Tyrosine Phosphorylation of Protein Kinase C
- Methods to Study Dephosphorylation of Protein Kinase C In Vivo
- Phosphopeptide-Specific Antibodies to Protein Kinase C
- Identifying Protein Kinase C Substrates
- Identifying Protein Kinase C Substrates
- A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases
- Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries
- Structural Analysis of Protein Kinase C
- Structural Analysis of Protein Kinase C
- Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms
- Crystallization of the Protein Kinase C? C1B Domain
- Methods for Detecting Binding Proteins
- Methods for Detecting Binding Proteins
- Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis
- Glutathione S-Transferase Pull-Down Assay
- Overlay Method for Detecting Protein-Protein Interactions
- An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates
- Functional Proteomic Analysis of the Protein Kinase C Signaling System
- Pharmacological Probes for Protein Kinase C
- Pharmacological Probes for Protein Kinase C
- Applications of Inhibitors for Protein Kinase C and Their Isoforms
- Phorbol Esters as Probes for the Study of Protein Kinase C Function
- Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs
- Genetic Approaches to Studying Protein Kinase C
- Genetic Approaches to Studying Protein Kinase C
- Animal Models in the Study of Protein Kinase C Isozymes
- Genetic Manipulation of Protein Kinase C In Vivo
- Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms
- Protein Kinase C in Disease
- Protein Kinase C in Disease
- Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models
- Alcohol Addiction.