Membrane Protein Protocols Expression, Purification, and Characterization /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Selinsky, Barry S. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2003.
Edición:1st ed. 2003.
Colección:Methods in Molecular Biology, 228
Materias:
Acceso en línea:https://doi.org/10.1385/159259400X
Tabla de Contenidos:
  • Expression of Membrane Proteins
  • Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
  • Dihydroorotate Dehydrogenase of Escherichia coli
  • A General Approach for Heterologous Membrane Protein Expression in Escherichia coli
  • Expression of Membrane-Bound Iron-Sulfur Proteins
  • Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris
  • Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
  • Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells
  • Detergent Selection in Membrane Protein Purification
  • Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
  • Solubilization of Chemokine Receptors from Cell Membranes
  • A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid
  • Membrane Protein Purification
  • Purification of Omp50, a Minor Porin of Campylobacter jejuni
  • Purification of Porins from Mycobacterium smegmatis
  • Isolation of the Melanocortin 5 Receptor
  • Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography
  • Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells
  • Purification of Pancreastatin Receptor from Rat Liver Membranes
  • Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
  • Isolation of Lipid Raft-Associated Proteolipids
  • Purification of Membrane-Bound Catechol-O-Methyltransferase
  • Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
  • Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase
  • Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
  • Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
  • Structural Analysis of Membrane Proteins
  • Crystallization in Lipidic Cubic Phases
  • Optical Biosensor Assay Using Retroviral Receptor Pseudotypes.