Mammalian Artificial Chromosomes Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Sgaramella, Vittorio. (Editor), Eridani, Sandro. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edition:1st ed. 2004.
Series:Methods in Molecular Biology, 240
Subjects:
Online Access:https://doi.org/10.1385/1592594344
Table of Contents:
  • From Natural to Artificial Chromosomes
  • Methodologies Applied to Domestic Animal Chromosomes
  • Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes
  • Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals
  • Centromeres and Neocentromeres
  • Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins
  • Telomere Length Analysis and In Vitro Telomerase Assay
  • Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice
  • Chromosome-Based Vectors for Mammalian Cells
  • Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor
  • Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis
  • Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice
  • Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications.