Methods of Protein Microcharacterization A Practical Handbook /

Bibliographic Details
Main Author: Shively, John E. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1986.
Edition:1st ed. 1986.
Series:Biological Methods
Subjects:
Online Access:https://doi.org/10.1007/978-1-59259-436-8
Table of Contents:
  • Microisolation Techniques
  • Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography
  • Reverse-Phase HPLC Isolation and Microsequence Analysis
  • Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis
  • Microamino Acid Analysis
  • Amino Acid Analysis With Postcolumn Fluorescent Derivatization
  • Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC
  • Amino Terminal Analysis
  • Manual Edman Sequencing System
  • Comparison of the Spinning Cup and Gas-Phase Instruments
  • Gas-Phase Protein/Peptide Sequencer
  • Design of a Multipurpose Sequencer
  • Solid-Phase Methods in Protein Microsequence Analysis
  • Analysis of Phenylthiohydantoin Amino Acids by HPLC
  • Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation
  • Carboxyl-Terminal Analysis
  • Microsequence Analysis by Enzymatic Methods
  • COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods
  • Mass Spectrometric Analysis of Polypeptides
  • Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry
  • Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.