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02982nam a22002895i 4500 |
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978-1-59259-490-0 |
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20190617192727.0 |
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cr nn 008mamaa |
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100301s1988 xxu| s |||| 0|eng d |
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|a 9781592594900
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| 024 |
7 |
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|a 10.1385/0896031268
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a New Protein Techniques
|c edited by John M. Walker.
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| 250 |
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|a 1st ed. 1988.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1988.
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| 300 |
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|a XV, 531 p. 33 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 3
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| 505 |
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|a Prevention of Unwanted Proteolysis -- The Bradford Method for Protein Quantitation -- Amino Acid Analysis by Precolumn Derivatization -- Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography -- Enzymatic Methods for Cleaving Proteins -- Chemical Cleavage of Proteins -- Chemical Modification of Proteins -- The Design, Preparation, and Use of Immunopurification Reagents -- Dye-Ligand Chromatography -- Aminohexyl-Sepharose Affinity Chromatography -- Purification of DNA-Dependent RNA Polymerase from Eubacteria -- Direct Immunoprecipitation of Protein -- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique -- Chromatofocusing -- Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels -- Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension -- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension -- Preparative Aspects of Immobilized pH Gradients -- Isoelectric Focusing Under Denaturing Conditions -- Computer Analysis of 2-D Electrophoresis Gels -- Two-Dimensional (Crossed) Immunoelectrophoresis -- Peptide Synthesis -- Synthesis of a Series of Analogous Peptides Using T-Bags -- Production of Antisera to Synthetic Peptides -- Production of Antibodies Using Proteins in Gel Bands -- Purification of Immunoglobulins Using Protein A-Sepharose -- Antibody-Enzyme Conjugate Formation -- Vacuum Blotting -- Blotting with Plate Electrodes -- Use of Dried Milk for Immunoblotting -- Immunodetection of Proteins on “Western” Blots Using 125I-Labeled Protein A -- Detection of Protein Blots Using the Avidin-Biotin System -- Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A -- Colloidal Gold for the Detection of Proteins on Blots and Immunoblots -- Enzyme Immobilization by Adsorption -- Enzyme Immobilization by Entrapment -- Enzyme Immobilization by Covalent Bonding -- Cell Immobilization.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
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|a Biochemistry, general.
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| 700 |
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|a Walker, John M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896031268
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