New Protein Techniques
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1988.
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| Edición: | 1st ed. 1988. |
| Colección: | Methods in Molecular Biology,
3 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896031268 |
Tabla de Contenidos:
- Prevention of Unwanted Proteolysis
- The Bradford Method for Protein Quantitation
- Amino Acid Analysis by Precolumn Derivatization
- Identification of N-Terminal Amino Acids by High-Performance Liquid Chromatography
- Enzymatic Methods for Cleaving Proteins
- Chemical Cleavage of Proteins
- Chemical Modification of Proteins
- The Design, Preparation, and Use of Immunopurification Reagents
- Dye-Ligand Chromatography
- Aminohexyl-Sepharose Affinity Chromatography
- Purification of DNA-Dependent RNA Polymerase from Eubacteria
- Direct Immunoprecipitation of Protein
- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
- Chromatofocusing
- Hybrid Isoelectric Focusing Using Mixed Synthetic-Carrier Ampholyte-Immobilized pH Gradient Gels
- Two-Dimensional Electrophoresis Using Immobilized pH Gradients in the First Dimension
- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
- Preparative Aspects of Immobilized pH Gradients
- Isoelectric Focusing Under Denaturing Conditions
- Computer Analysis of 2-D Electrophoresis Gels
- Two-Dimensional (Crossed) Immunoelectrophoresis
- Peptide Synthesis
- Synthesis of a Series of Analogous Peptides Using T-Bags
- Production of Antisera to Synthetic Peptides
- Production of Antibodies Using Proteins in Gel Bands
- Purification of Immunoglobulins Using Protein A-Sepharose
- Antibody-Enzyme Conjugate Formation
- Vacuum Blotting
- Blotting with Plate Electrodes
- Use of Dried Milk for Immunoblotting
- Immunodetection of Proteins on “Western” Blots Using 125I-Labeled Protein A
- Detection of Protein Blots Using the Avidin-Biotin System
- Detection of Protein Blots Using Enzyme-Linked Second Antibodies or Protein A
- Colloidal Gold for the Detection of Proteins on Blots and Immunoblots
- Enzyme Immobilization by Adsorption
- Enzyme Immobilization by Entrapment
- Enzyme Immobilization by Covalent Bonding
- Cell Immobilization.