Protocols in Human Molecular Genetics

Bibliographic Details
Main Author: Mathew, Christopher G. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1992.
Edition:1st ed. 1992.
Series:Methods in Molecular Biology, 9
Subjects:
Online Access:https://doi.org/10.1385/0896032051
Table of Contents:
  • The Polymerase Chain Reaction
  • Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction
  • Direct Sequencing of PCR-Amplified DNA
  • Rapid DNA Sequence Analysis Using Fluorescent Labels
  • Detection of Mutations in DNA and RNA by Chemical Cleavage
  • Rapid Methods for Detection of Polymorphic Markers in Genomic DNA
  • The Analysis of Point Mutations Using Synthetic Oligonucleotide Probes
  • Detection of Mutations by the Amplification Refractory Mutation System (ARMS)
  • Automated Gene Detection Using the Oligonucleotide Ligation Assay
  • Detection of Point Mutations by Denaturing-Gradient Gel Electrophoresis
  • The Detection and Mapping of Point Mutations by RNase A Cleavage
  • Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments
  • Extraction and Enzymatic Amplification of DNA from Paraffin-Embedded Specimens
  • The Use of the Polymerase Chain Reaction in the Mapping of Human Genes Using Somatic Cell Hybrids
  • The Southern Blot
  • The Detection of Specific DNA Sequences by Enhanced Chemiluminescence
  • Pulsed-Field Gel Electrophoresis
  • Cloning from Gels Following Pulse-Field Gel Electrophoresis
  • Yeast Artificial-Chromosome (YAC) Cloning Systems
  • Gene Targeting for Somatic Cell Manipulation
  • In Situ Hybridization of Chromosomes
  • DNA Fingerprinting Analysis
  • DNA Fingerprinting and Forensic Medicine
  • The Detection of Point Mutations in Hemoglobin Defects Using Allele-Specific Oligonucleotide Probes
  • Detection of Gene Deletions Using Multiplex Polymerase Chain Reactions
  • Application of Pulsed-Field Gel Electrophoresis to Genetic Diagnosis
  • Molecular Diagnostics of Cancer
  • The Detection of Latent Virus Infection by Polymerase Chain Reaction
  • Mapping Inherited Diseases by Linkage Analysis
  • Diagnosis of Genetic Disorders with Linked DNA Markers
  • Software for Genetic Linkage Analysis
  • Creating Animal Models of Genetic Disease
  • Molecular Biology and Medicine.