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03020nam a22003015i 4500 |
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978-1-59259-499-3 |
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20190617194209.0 |
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cr nn 008mamaa |
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100301s1992 xxu| s |||| 0|eng d |
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|a 9781592594993
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| 024 |
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|a 10.1385/0896032299
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Burmeister, Margit.
|e author.
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| 245 |
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|a Pulsed-Field Gel Electrophoresis
|b Protocols, Methods, and Theories /
|c by Margit Burmeister, Levy Ulanovsky.
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| 250 |
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|a 1st ed. 1992.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1992.
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| 300 |
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|a XIII, 481 p. 99 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 12
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| 505 |
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|a Electrophoretic Techniques -- Field-Inversion Gel Electrophoresis -- Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF) -- Separation and Size Determination of DNA over a 10–,200 kbp Range -- PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE) -- Transverse Alternating-Field Electrophoresis -- Rotating Field Gel Electrophoresis (ROFE) -- Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Auxiliary Methods -- Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis -- Encapsulation of Cells in Agarose Beads -- Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis -- Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases -- Desktop Digital Imaging -- Biological Applications -- Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE) -- Protozoan Genomes -- Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis -- Analysis of Yeast Artificial Chromosome Clones -- Strategies for Mapping Large Regions of Mammalian Genomes -- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA -- Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA -- Construction of Lambda Libraries from Large PFGE Fragments -- In-Gel Detection of DNA -- Theories and Observations -- Theories of Pulsed-Field Gel Electrophoresis -- The Dynamics of DNA Gel Electrophoresis -- Orientation of the Agarose Matrix by Pulsed Electric Fields -- Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis -- Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Ulanovsky, Levy.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896032299
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