Pulsed-Field Gel Electrophoresis Protocols, Methods, and Theories /

Pulsed-Field Gel Electrophoresis Protocols, Methods, and Theories /

Detalles Bibliográficos
Autores principales: Burmeister, Margit. (Autor), Ulanovsky, Levy. (Autor)
Autor Corporativo: SpringerLink (Online service)
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1992.
Edición:1st ed. 1992.
Colección:Methods in Molecular Biology, 12
Materias:
Biochemistry.
Biochemistry, general.
Acceso en línea:https://doi.org/10.1385/0896032299
Tabla de Contenidos:
  • Electrophoretic Techniques
  • Field-Inversion Gel Electrophoresis
  • Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF)
  • Separation and Size Determination of DNA over a 10–,200 kbp Range
  • PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)
  • Transverse Alternating-Field Electrophoresis
  • Rotating Field Gel Electrophoresis (ROFE)
  • Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE)
  • Auxiliary Methods
  • Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis
  • Encapsulation of Cells in Agarose Beads
  • Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE)
  • Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis
  • Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases
  • Desktop Digital Imaging
  • Biological Applications
  • Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE)
  • Protozoan Genomes
  • Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis
  • Analysis of Yeast Artificial Chromosome Clones
  • Strategies for Mapping Large Regions of Mammalian Genomes
  • Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA
  • Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA
  • Construction of Lambda Libraries from Large PFGE Fragments
  • In-Gel Detection of DNA
  • Theories and Observations
  • Theories of Pulsed-Field Gel Electrophoresis
  • The Dynamics of DNA Gel Electrophoresis
  • Orientation of the Agarose Matrix by Pulsed Electric Fields
  • Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis
  • Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

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