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03777nam a22003015i 4500 |
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978-1-59259-505-1 |
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20191026142233.0 |
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cr nn 008mamaa |
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100301s1993 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595051
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| 024 |
7 |
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|a 10.1385/0896032450
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Murphy, David.
|e author.
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| 245 |
1 |
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|a Transgenesis Techniques
|b Principles and Protocols /
|c by David Murphy, David A. Carter.
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| 250 |
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|a 1st ed. 1993.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1993.
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| 300 |
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|a XII, 467 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 18
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| 505 |
0 |
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|a and Topical Reviews -- to Transgenesis -- Transgenic Rodents and the Study of the Central Nervous System -- Transgenic Animals and the Study of Cancer -- Transgenic Animals and the Study of the Immune System -- The Commercial and Agricultural Applications of Animal Transgenesis -- Transgenesis in Invertebrate and Lower Vertebrate Species -- P Element-Mediated Germ-Line Transformation of Drosophila -- Gene Transfer by Microinjection in the Zebra Fish Brachydanio rerio -- Transgenic Induction in Salmonid and Tilapia Fish -- Transgenesis in the Mouse -- An Overview of Transgenic Mouse Production -- Transgene Design -- Isolation of DNA Fragments for Microinjection -- Establishing a Colony for Efficient Production of Transgenic Mice -- Mating Mice -- Anesthetizing Mice -- Vasectomizing a Mouse -- Preparation of Culture Media for Fertilized One-Cell Mouse Eggs -- Collection of Fertilized One-Cell Mouse Eggs for Microinjection -- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs -- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs -- Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer -- Caesarean Section and Fostering -- In vitro Fertilization of Mouse Eggs -- The In Vitro Isolation of Murine Embryonic Stem Cells -- Production of Chimeras Derived from Murine Embryonic Stem Cells -- Cryopreservation of Transgenic Mouse Lines -- Transgenesis in the Rat -- to the Physiology and Husbandry of the Rat -- Mating of Rats -- Vasectomy of the Male Rat -- Harvest of Fertilized One-Cell Rat Eggs -- Implantation of Microinjected Eggs -- Cryopreservation of Transgenic Rat Lines -- Transgenesis in a Domestic Species-The Sheep -- Production of Transgenic Sheep -- Cryopreservation of Transgenic Sheep Lines -- Genomic Analysis of Transgenic Animals -- Isolation of Genomic DNA from Tail Tissue -- Slot Blotting of Genomic Tail DNA -- Genomic Analysis of Transgenic Animals by the Polymerase Chain Reaction -- Genomic Analysis of Transgenic Animals -- Expression Analysis of Transgenic Animals -- The Preparation of RNA from the Tissues of Transgenic Animals -- Northern Blotting -- Rna slot Blotting -- Analysis of Gene Expression by PCR -- Nuclear Run-On Analysis of Transcription -- S1 Nuclease Protection Mapping -- Primer Extension -- In Situ Hybridization Analysis of Transgenic Tissues -- Immunohistochemical Analysis of Transgene Expression -- Postmortem Examination of Transgenic Mice -- Histological Examination of Transgenic Mice -- Reporter Enzyme Assays -- Restriction Endonuclease Digestion of DNA -- Agarose Gel Electrophoresis -- Working with Radioactivity in the Transgenic Animal Laboratory -- Random Primed Labeling of DNA -- Filter Hybridization -- DNA Transfection.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Carter, David A.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896032450
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