Transgenesis Techniques Principles and Protocols /
| Autores principales: | , |
|---|---|
| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1993.
|
| Edición: | 1st ed. 1993. |
| Colección: | Methods in Molecular Biology,
18 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896032450 |
Tabla de Contenidos:
- and Topical Reviews
- to Transgenesis
- Transgenic Rodents and the Study of the Central Nervous System
- Transgenic Animals and the Study of Cancer
- Transgenic Animals and the Study of the Immune System
- The Commercial and Agricultural Applications of Animal Transgenesis
- Transgenesis in Invertebrate and Lower Vertebrate Species
- P Element-Mediated Germ-Line Transformation of Drosophila
- Gene Transfer by Microinjection in the Zebra Fish Brachydanio rerio
- Transgenic Induction in Salmonid and Tilapia Fish
- Transgenesis in the Mouse
- An Overview of Transgenic Mouse Production
- Transgene Design
- Isolation of DNA Fragments for Microinjection
- Establishing a Colony for Efficient Production of Transgenic Mice
- Mating Mice
- Anesthetizing Mice
- Vasectomizing a Mouse
- Preparation of Culture Media for Fertilized One-Cell Mouse Eggs
- Collection of Fertilized One-Cell Mouse Eggs for Microinjection
- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs
- Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs
- Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer
- Caesarean Section and Fostering
- In vitro Fertilization of Mouse Eggs
- The In Vitro Isolation of Murine Embryonic Stem Cells
- Production of Chimeras Derived from Murine Embryonic Stem Cells
- Cryopreservation of Transgenic Mouse Lines
- Transgenesis in the Rat
- to the Physiology and Husbandry of the Rat
- Mating of Rats
- Vasectomy of the Male Rat
- Harvest of Fertilized One-Cell Rat Eggs
- Implantation of Microinjected Eggs
- Cryopreservation of Transgenic Rat Lines
- Transgenesis in a Domestic Species-The Sheep
- Production of Transgenic Sheep
- Cryopreservation of Transgenic Sheep Lines
- Genomic Analysis of Transgenic Animals
- Isolation of Genomic DNA from Tail Tissue
- Slot Blotting of Genomic Tail DNA
- Genomic Analysis of Transgenic Animals by the Polymerase Chain Reaction
- Genomic Analysis of Transgenic Animals
- Expression Analysis of Transgenic Animals
- The Preparation of RNA from the Tissues of Transgenic Animals
- Northern Blotting
- Rna slot Blotting
- Analysis of Gene Expression by PCR
- Nuclear Run-On Analysis of Transcription
- S1 Nuclease Protection Mapping
- Primer Extension
- In Situ Hybridization Analysis of Transgenic Tissues
- Immunohistochemical Analysis of Transgene Expression
- Postmortem Examination of Transgenic Mice
- Histological Examination of Transgenic Mice
- Reporter Enzyme Assays
- Restriction Endonuclease Digestion of DNA
- Agarose Gel Electrophoresis
- Working with Radioactivity in the Transgenic Animal Laboratory
- Random Primed Labeling of DNA
- Filter Hybridization
- DNA Transfection.