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02833nam a22003015i 4500 |
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978-1-59259-506-8 |
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20191026171851.0 |
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cr nn 008mamaa |
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100301s1993 xxu| s |||| 0|eng d |
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|a 9781592595068
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| 024 |
7 |
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|a 10.1385/0896032361
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Graham, John M.
|e author.
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| 245 |
1 |
0 |
|a Biomembrane Protocols
|b I. Isolation and Analysis /
|c by John M. Graham, Joan A. Higgins.
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| 250 |
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|a 1st ed. 1993.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1993.
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| 300 |
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|a X, 314 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 19
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| 505 |
0 |
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|a The Identification of Subcellular Fractions from Mammalian Cells -- The Isolation of Nuclei and Nuclear Membranes from Rat Liver -- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver -- Continuous-Flow Electrophoresis -- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells -- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver -- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients -- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components -- Isolation of Membranes from Tissue Culture Cells -- The Isolation of Membranes from Bacteria -- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts -- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells -- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption -- Separation and Analysis of Phospholipids by Thin Layer Chromatography -- Analysis of Phospholipids by High Performance Liquid Chromatography -- Measurement of Cholesterol in Membranes -- Analysis of Fatty Acids by Gas Liquid Chromatography -- Chemical Assays for Proteins -- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis -- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins -- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase -- The Isolation of Membrane Proteoglycans -- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans -- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration -- The Extraction and Analysis of Glycosphingolipids.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Higgins, Joan A.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032361
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