Biomembrane Protocols I. Isolation and Analysis /

Bibliographic Details
Main Authors: Graham, John M. (Author), Higgins, Joan A. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1993.
Edition:1st ed. 1993.
Series:Methods in Molecular Biology, 19
Subjects:
Online Access:https://doi.org/10.1385/0896032361
Table of Contents:
  • The Identification of Subcellular Fractions from Mammalian Cells
  • The Isolation of Nuclei and Nuclear Membranes from Rat Liver
  • Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver
  • Continuous-Flow Electrophoresis
  • Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
  • Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver
  • The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients
  • The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components
  • Isolation of Membranes from Tissue Culture Cells
  • The Isolation of Membranes from Bacteria
  • Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts
  • Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
  • Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption
  • Separation and Analysis of Phospholipids by Thin Layer Chromatography
  • Analysis of Phospholipids by High Performance Liquid Chromatography
  • Measurement of Cholesterol in Membranes
  • Analysis of Fatty Acids by Gas Liquid Chromatography
  • Chemical Assays for Proteins
  • Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis
  • Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence
  • Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins
  • Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase
  • The Isolation of Membrane Proteoglycans
  • Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans
  • Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
  • The Extraction and Analysis of Glycosphingolipids.