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02820nam a22003015i 4500 |
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978-1-59259-510-5 |
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20191026142224.0 |
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cr nn 008mamaa |
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100301s1993 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595105
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| 024 |
7 |
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|a 10.1385/0896032485
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Griffin, Annette M.
|e author.
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| 245 |
1 |
0 |
|a DNA Sequencing Protocols
|c by Annette M. Griffin, Hugh G. Griffin.
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| 250 |
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|a 1st ed. 1993.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1993.
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| 300 |
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|a XII, 392 p. 32 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 23
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| 505 |
0 |
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|a DNA Sequencing -- M13 Cloning Vehicles -- Cloning into M13 -- Transfection of E. coli with M13 DNA -- M13 Phage Growth and Single-Strand DNA Preparation -- M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays -- Generation of Random Fragments by Sonication -- Generation of a Nested Set of Deletions Using Exonuclease III -- Sequential Deletions of Single-Stranded DNA -- Subcloning for DNA Sequencing -- Sequencing Using Custom Designed Oligonucleotides -- Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1 -- Dideoxy Sequencing Reactions Using T7 Polymerase -- Dideoxy Sequencing Reactions Using Sequenase Version 2.0 -- Dideoxy Sequencing Reactions Using Taq Polymerase -- Pouring Linear and Buffer-Gradient Sequencing Gels -- Electrophoresis of Sequence Reaction Samples -- Plasmid Sequencing -- Plasmid Sequencing -- Direct Sequencing of Inserts Cloned into Lambda Vectors -- Cosmid Sequencing -- Genomic Sequencing -- Sequencing of Double-Stranded PCR Products -- Sequencing Double-Stranded Linear DNA with Sequenase and [?-35S] d.ATP -- Solid Phase PCR Sequencing of Biotinylated Products -- Cycle Sequencing -- Direct Blotting Electrophoresis -- Multipllex DNA Sequencing -- DNA Sequencing by Chemiluminescent Detection -- Reverse Sequencing of M13 Cloned DNA -- Terminal Labeling of DNA for Maxam and Gilbert Sequencing -- DNA Sequencing the Chemical Method -- DNA Sequencing by Chemical Degradation Using One, Two, and Four Different Fluorophores -- Linear Amplification Sequencing with Dye Terminators -- Sequencing Reactions for the Applied Biosystems 373AAutomated DNA Sequencer -- Sequencing Reactions for ALF (EMBL) Automated DNA Sequencer -- Sequencing Using the Du Pont Genesis™ 2000 DNA Analysis System -- The Use of Robotic Workstations in DNA Sequencing.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Griffin, Hugh G.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032485
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