DNA Sequencing Protocols
| Autores principales: | , |
|---|---|
| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1993.
|
| Edición: | 1st ed. 1993. |
| Colección: | Methods in Molecular Biology,
23 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896032485 |
Tabla de Contenidos:
- DNA Sequencing
- M13 Cloning Vehicles
- Cloning into M13
- Transfection of E. coli with M13 DNA
- M13 Phage Growth and Single-Strand DNA Preparation
- M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays
- Generation of Random Fragments by Sonication
- Generation of a Nested Set of Deletions Using Exonuclease III
- Sequential Deletions of Single-Stranded DNA
- Subcloning for DNA Sequencing
- Sequencing Using Custom Designed Oligonucleotides
- Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
- Dideoxy Sequencing Reactions Using T7 Polymerase
- Dideoxy Sequencing Reactions Using Sequenase Version 2.0
- Dideoxy Sequencing Reactions Using Taq Polymerase
- Pouring Linear and Buffer-Gradient Sequencing Gels
- Electrophoresis of Sequence Reaction Samples
- Plasmid Sequencing
- Plasmid Sequencing
- Direct Sequencing of Inserts Cloned into Lambda Vectors
- Cosmid Sequencing
- Genomic Sequencing
- Sequencing of Double-Stranded PCR Products
- Sequencing Double-Stranded Linear DNA with Sequenase and [?-35S] d.ATP
- Solid Phase PCR Sequencing of Biotinylated Products
- Cycle Sequencing
- Direct Blotting Electrophoresis
- Multipllex DNA Sequencing
- DNA Sequencing by Chemiluminescent Detection
- Reverse Sequencing of M13 Cloned DNA
- Terminal Labeling of DNA for Maxam and Gilbert Sequencing
- DNA Sequencing the Chemical Method
- DNA Sequencing by Chemical Degradation Using One, Two, and Four Different Fluorophores
- Linear Amplification Sequencing with Dye Terminators
- Sequencing Reactions for the Applied Biosystems 373AAutomated DNA Sequencer
- Sequencing Reactions for ALF (EMBL) Automated DNA Sequencer
- Sequencing Using the Du Pont Genesis™ 2000 DNA Analysis System
- The Use of Robotic Workstations in DNA Sequencing.