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02926nam a22003015i 4500 |
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978-1-59259-514-3 |
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20191026142650.0 |
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cr nn 008mamaa |
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100301s1994 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595143
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| 024 |
7 |
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|a 10.1385/0896032507
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Graham, John M.
|e author.
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| 245 |
1 |
0 |
|a Biomembrane Protocols
|b II. Architecture and Function /
|c by John M. Graham, Joan A. Higgins.
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| 250 |
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|a 1st ed. 1994.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1994.
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| 300 |
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|a XIII, 362 p. 7 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 27
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| 505 |
0 |
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|a Crystallization of Membrane Proteins for X-Ray Analysis -- Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination -- Biochemical Methods to Determine Cell-Surface Topography -- Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins -- The Production of Monoclonal Antibodies to Membrane Proteins -- Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles -- Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes -- Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools -- Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry -- Fluorescent Glycerolipid Probes -- Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids -- Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes -- Extraction and Assay of Cyclic Nucleotides -- Analysis of G-Proteins Regulating Signal Transduction Pathways -- Assay of Protein Kinases and Protein Phosphorylation -- Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures -- Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography -- Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes -- Membrane Permeabilization with Bacterial Toxins -- Measurement of Ion Fluxes and pH Gradients Across Cell Membranes -- Ligand Binding and Processing -- The Binding of Protein-Ligands to Cell-Surface Receptors.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Higgins, Joan A.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032507
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