Biomembrane Protocols II. Architecture and Function /
| Autores principales: | , |
|---|---|
| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1994.
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| Edición: | 1st ed. 1994. |
| Colección: | Methods in Molecular Biology,
27 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896032507 |
Tabla de Contenidos:
- Crystallization of Membrane Proteins for X-Ray Analysis
- Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination
- Biochemical Methods to Determine Cell-Surface Topography
- Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins
- The Production of Monoclonal Antibodies to Membrane Proteins
- Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
- Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy
- Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy
- Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes
- Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools
- Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry
- Fluorescent Glycerolipid Probes
- Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
- Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes
- Extraction and Assay of Cyclic Nucleotides
- Analysis of G-Proteins Regulating Signal Transduction Pathways
- Assay of Protein Kinases and Protein Phosphorylation
- Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures
- Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography
- Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes
- Membrane Permeabilization with Bacterial Toxins
- Measurement of Ion Fluxes and pH Gradients Across Cell Membranes
- Ligand Binding and Processing
- The Binding of Protein-Ligands to Cell-Surface Receptors.