Protocols for Gene Analysis
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1994.
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| Edition: | 1st ed. 1994. |
| Series: | Methods in Molecular Biology,
31 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896032582 |
Table of Contents:
- Basic Recombinant Dna Techniques
- Transformation of Bacteria by Electroporation
- Direct Cloning of Agtll cDNA Inserts Into a Plasmid Vector
- PCR Cloning Using T-Vectors
- Thermal Cycle Dideoxy DNA Sequencing
- In Vitro Mutagenesis
- Ordered Deletions Using Exonuclease III
- Site-Directed Mutagenesis Using a Double-Stranded DNA Template
- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation
- Construction of Linker-Scanning Mutations Using the Polymerase Chain Reaction
- Localized Random Polymerase Chain Reaction Mutagenesis
- Genomic Structure
- The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells
- Physical Mapping of the Human Genome by Pulsed Field Gel Electrophoresis
- Field Inversion Gel Electrophoresis
- Enhanced Chemiluminescent Detection of Horseradish Peroxidase Labeled Probes
- Nonradioactive Oligonucleotide Probe Labeling
- Analysis of DNA Restriction Enzyme Digests by Two-Dimensional Electrophoresis in Agarose Gels
- Inverse Polymerase Chain Reaction
- Sequence Variations
- Use of Silver Staining to Detect Nucleic Acids
- A Nonradioactive Method for the Detection of Single-Strand Conformational Polymorphisms (SSCP)
- Temperature Gradient Gel Electrophoresis (TGGE) for the Detection of Polymorphic DNA and RNA
- TGGE in Quantitative PCR of DNA and RNA
- The PGK-PCR Clonality Assay (PPCA)
- Direct Sequencing of PCR Products
- Gene Expression
- The Use of Riboprobes for the Analysis of Gene Expression
- Quantification of Absolute Amounts of Cellular Messenger RNA by RNA-Excess Solution Hybridization
- Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay
- An RNA Polymerase II In Vitro Transcription System
- SI Mapping Using Single-Stranded DNA Probes
- Single Primer-Mediated Polymerase Chain Reaction
- Protein-Dna Interactions
- In Vivo DNA Footprinting by Linear Amplification
- DNA Photofootprinting with Rh(phi)2bpy3+
- The Gel Retardation Assay
- The Southwestern Assay
- Cloning DNA Binding Proteins from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes
- Protein Function
- 6xffis-Ni-NTA Chromatography as a Superior Technique in Recombinant Protein Expression/Purification
- Production of 35S-Labeled Proteins in E. coli and Their Use as Molecular Probes
- Preparation and Ligand Screening of a ?gt11 Lysogen Library.