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03368nam a22002895i 4500 |
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978-1-59259-521-1 |
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20190618091210.0 |
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cr nn 008mamaa |
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100301s1995 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595211
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| 024 |
7 |
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|a 10.1385/089603285X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Immunocytochemical Methods and Protocols
|c edited by Lorette C. Javois.
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| 250 |
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|a 1st ed. 1995.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1995.
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| 300 |
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|a XII, 435 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 34
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|a Overview of Antibody Use in Immunocytochemistry -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration -- Purification of Antibodies Using Ion-Exchange Chromatography -- Purification of Antibodies Using Affinity Chromatography -- Purification of Antibodies Using Protein A-Sepharose and FPLC -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Overview of Cell Fixation and Permeabilization -- Preparation of Frozen Sections for Analysis -- Processing of Cell-Touch Preparations -- Processing of Cytological Specimens -- Processing of Tissue-Culture Cells -- Processing of Tissue Specimens -- Overview of Fluorophores -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method -- The Avidin-Biotin Complex(ABC) Method -- Labeled Avidin Binding (LAB) Method* -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- ELISA on Attachment-Dependent or Suspension Grown Cells -- Use of Immunogold with Silver Enhancement -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Assay for Phagocytosis -- Assay for Filamentous Actin -- Assay for Chemoattractant Binding -- Assay for Oxidative Metabolism -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-Embedding Labeling Methods -- Postembedding Labeling Methods -- Overview of Fluorescence Photomicrography -- Overview of Fluorescence Analysis with the Confocal Microscope -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Nucleic Acid Immunocytochemistry -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes -- Overview of Automated Immunostainers -- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
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|a Javois, Lorette C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603285X
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