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02870nam a22002895i 4500 |
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978-1-59259-524-2 |
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20190618091317.0 |
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cr nn 008mamaa |
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100301s1995 xxu| s |||| 0|eng d |
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|a 9781592595242
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| 024 |
7 |
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|a 10.1385/0896032884
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a In Vitro Transcription and Translation Protocols
|c edited by Martin J. Tymms.
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| 250 |
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|a 1st ed. 1995.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1995.
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| 300 |
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|a XII, 432 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 37
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|a Transcription In Vitro Using Bacteriophage RNA Polymerases -- Subtraction Hybridization cDNA Libraries -- Quantitative Measurement of mRNA Using the RNase Protection Assay -- Assembly and Transcription of Chromatin Templates Using RNA Polymerase III -- Antisense Affinity Depletion of RNP Particles -- In Vitro mRNA Editing Using S100 Extracts -- An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA -- In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Breast Carcinoma Cells -- Plastid In Vitro Transcription -- Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Early Embryos to Study the Gain-and Loss-of-Function of Genes -- mRNA Translation in Xenopus Oocytes -- Transcription System Using a HeLa Cell Mitochondrial Lysate -- The Xenopus Egg Extract Translation System -- In Vitro Translation Using Rabbit Reticulocyte Lysate -- An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori -- Translation Using a Wheat-Germ Extract -- In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani -- Preparation and Use of E. coli S-30 Extracts -- Posttranslational Transport and In Thylakoido Integration of Plastid Protein -- Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro -- Structure-Function studies based on in vitro expression -- ?-Like Plastid Transcription Factors -- Preparation of HeLa Nuclear Extracts -- Purification of the human TATA-Binding Protein, TBP -- DNA-Binding studies using in vitro synthesized myb proteins -- DNase i footprinting using PCR-generated end-labeled DNA probes -- Gene Isolation by Screening ?gt11 Expression Libraries with DNA Binding-Site Probes -- Transcriptional activation analysis by the Chloramphenicol Acetyl Transferase (CAT) enzyme assay.
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| 650 |
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|a Cytology.
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| 650 |
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|a Cell Biology.
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| 700 |
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|a Tymms, Martin J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896032884
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