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03011nam a22002895i 4500 |
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978-1-59259-532-7 |
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20190618101546.0 |
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cr nn 008mamaa |
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100301s1995 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595327
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| 024 |
7 |
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|a 10.1385/0896033082
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Davis, William C.
|e author.
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| 245 |
1 |
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|a Monoclonal Antibody Protocols
|c by William C. Davis.
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| 250 |
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|a 1st ed. 1995.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1995.
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| 300 |
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|a X, 264 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 45
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| 505 |
0 |
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|a Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies -- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro -- Culture Conditions that Optimize Outgrowth of Hybridomas -- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies -- Production of Stable Bovine-Murine Interspecies Hybrids -- Production of Monoclonal Antibodies in Swine -- Production of Monoclonal Antibodies in Horses -- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies -- Rapid Isotyping of Mouse Monoclonal Antibodies -- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media -- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay -- Use of Monoclonal Antibodies in Immunohistochemistry -- Use of Monoclonal Antibodies in Immunoelectron Microscopy -- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection -- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry -- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules -- In Vivo and In Vitro Production of Monoclonal Antibodies -- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose -- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies -- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium -- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography -- Coupling of Monoclonal Antibodies with Fluorophores -- Coupling of Monoclonal Antibodies with Biotin -- Coupling of Monoclonal Antibodies with Enzymes -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection -- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896033082
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