Monoclonal Antibody Protocols
| Autor principal: | |
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| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1995.
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| Edición: | 1st ed. 1995. |
| Colección: | Methods in Molecular Biology,
45 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896033082 |
Tabla de Contenidos:
- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies
- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro
- Culture Conditions that Optimize Outgrowth of Hybridomas
- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies
- Production of Stable Bovine-Murine Interspecies Hybrids
- Production of Monoclonal Antibodies in Swine
- Production of Monoclonal Antibodies in Horses
- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies
- Rapid Isotyping of Mouse Monoclonal Antibodies
- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media
- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
- Use of Monoclonal Antibodies in Immunohistochemistry
- Use of Monoclonal Antibodies in Immunoelectron Microscopy
- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection
- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry
- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules
- In Vivo and In Vitro Production of Monoclonal Antibodies
- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose
- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies
- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium
- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
- Coupling of Monoclonal Antibodies with Fluorophores
- Coupling of Monoclonal Antibodies with Biotin
- Coupling of Monoclonal Antibodies with Enzymes
- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection
- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.