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03118nam a22002895i 4500 |
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978-1-59259-540-2 |
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20191027041518.0 |
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cr nn 008mamaa |
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100301s1996 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595402
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| 024 |
7 |
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|a 10.1385/0896033198
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Yeast Protocols
|b Methods in Cell and Molecular Biology /
|c edited by Ivor H. Evans.
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| 250 |
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|a 1st ed. 1996.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1996.
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| 300 |
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|a XIII, 433 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 53
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| 505 |
0 |
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|a Isolation and Identification of Yeasts from Natural Habitats -- Maintenance and Culture of Yeasts -- Mutagenesis in Yeast -- Rare-Mating and Cytoduction in Saccharomyces cerevisiae -- Protoplast Fusion in Saccharomyces cerevisiae -- Meiotic Analysis -- Ascus Dissection -- Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis -- Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques -- Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy -- Isolation of Yeast DNA -- Isolation of Mitochondrial DNA -- Isolation of Yeast Plasma Membranes -- The Extraction and Analysis of Sterols from Yeast -- Peroxisome Isolation -- Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers -- Biolistic Transformation of Yeasts -- Genomic Yeast DNA Clone Banks -- Yeast Colony Hybridization -- Determination of Plasmid Copy Number in Yeast -- Determination of Chromosome Ploidy in Yeast -- Chromosome Engineering in Yeast with a Site-Specific Recombination System from a Heterologous Yeast Plasmid -- Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae -- Reporter Gene Systems for Assaying Gene Expression in Yeast -- Preparation and Use of Yeast Cell-Free Translation Lysate -- Electrophoretic Analysis of Yeast Proteins -- Preparation of Total RNA -- mRNA Abundance and Half-Life Measurements -- Polysome Analysis -- Induction of Heat Shock Proteins and Thermotolerance -- Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes -- Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis -- Application of Killer Toxins in Stepwise Selection of Hybrids and Cybrids Obtained by Induced Protoplast Fusion -- Killer Plaque Technique for Selecting Hybrids and Cybrids Obtained by Induced Protoplast Fusion -- Genotoxicity Testing in Schizosaccharomyces pombe -- Purification and Quantification of Saccharomyces cerevisiae Cytochrome P450 -- Calorimetry of Whole Yeast Cells -- Light Microscopy Methods -- Fluorescence Microscopy Methods -- Immunoelectron Microscopy.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Evans, Ivor H.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033198
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