Yeast Protocols Methods in Cell and Molecular Biology /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1996.
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| Edición: | 1st ed. 1996. |
| Colección: | Methods in Molecular Biology,
53 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896033198 |
Tabla de Contenidos:
- Isolation and Identification of Yeasts from Natural Habitats
- Maintenance and Culture of Yeasts
- Mutagenesis in Yeast
- Rare-Mating and Cytoduction in Saccharomyces cerevisiae
- Protoplast Fusion in Saccharomyces cerevisiae
- Meiotic Analysis
- Ascus Dissection
- Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis
- Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques
- Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy
- Isolation of Yeast DNA
- Isolation of Mitochondrial DNA
- Isolation of Yeast Plasma Membranes
- The Extraction and Analysis of Sterols from Yeast
- Peroxisome Isolation
- Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers
- Biolistic Transformation of Yeasts
- Genomic Yeast DNA Clone Banks
- Yeast Colony Hybridization
- Determination of Plasmid Copy Number in Yeast
- Determination of Chromosome Ploidy in Yeast
- Chromosome Engineering in Yeast with a Site-Specific Recombination System from a Heterologous Yeast Plasmid
- Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae
- Reporter Gene Systems for Assaying Gene Expression in Yeast
- Preparation and Use of Yeast Cell-Free Translation Lysate
- Electrophoretic Analysis of Yeast Proteins
- Preparation of Total RNA
- mRNA Abundance and Half-Life Measurements
- Polysome Analysis
- Induction of Heat Shock Proteins and Thermotolerance
- Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes
- Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis
- Application of Killer Toxins in Stepwise Selection of Hybrids and Cybrids Obtained by Induced Protoplast Fusion
- Killer Plaque Technique for Selecting Hybrids and Cybrids Obtained by Induced Protoplast Fusion
- Genotoxicity Testing in Schizosaccharomyces pombe
- Purification and Quantification of Saccharomyces cerevisiae Cytochrome P450
- Calorimetry of Whole Yeast Cells
- Light Microscopy Methods
- Fluorescence Microscopy Methods
- Immunoelectron Microscopy.