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02375nam a22002895i 4500 |
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978-1-59259-545-7 |
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20191027051510.0 |
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cr nn 008mamaa |
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100301s1996 xxu| s |||| 0|eng d |
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|a 9781592595457
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| 024 |
7 |
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|a 10.1385/0896033368
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Protein Purification Protocols
|c edited by Shawn Doonan.
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| 250 |
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|a 1st ed. 1996.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1996.
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| 300 |
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|a X, 406 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 59
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| 505 |
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|a General Strategies -- Preparation of Extracts from Animal Tissues -- Protein Extraction from Plant Tissues -- Extraction of Recombinant Protein from Bacteria -- Protein Extraction from Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional Precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography (FPLC) Methods -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent from Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins from Polyacrylamide Gels -- Electroblotting of Proteins from Polyacrylamide Gels -- High-Performance Electrophoresis Chromatography -- Practical Column Chromatography.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Doonan, Shawn.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896033368
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