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02837nam a22003255i 4500 |
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978-1-59259-555-6 |
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20191027061622.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595556
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| 024 |
7 |
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|a 10.1385/089603383X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a cDNA Library Protocols
|c edited by Ian G. Cowell, Caroline A. Austin.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a X, 321 p. 28 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 69
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| 505 |
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|a cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR -- Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations -- Isolation of Messenger RNA from Plant Tissues -- cDNA Library Construction for the Lambda ZAP®-Based Vectors -- Clone Excision Methods for the Lambda ZAP®-Based Vectors -- Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs -- Inverse PCR Approach to Cloning cDNA Ends -- Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers -- Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads -- Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli -- The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences -- Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides -- Immunological Screening of ?-Phage cDNA Expression Libraries -- Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes -- Protein Interaction Cloning by Far-Western Screening of ?-Phage cDNA Expression Libraries -- Yeast Two-Hybrid Library Screening -- Signal Sequence Trap -- Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors -- Expression and Preparation of Fusion Proteins from Recombinant ?gt1.1 Phages -- Computer Analysis of Cloned Sequences -- The TIGR Human cDNA Database -- Searching the dbEST Database -- Reference cDNA Library Facilities Available from European Sources.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Immunology.
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| 700 |
1 |
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|a Cowell, Ian G.
|e editor.
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| 700 |
1 |
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|a Austin, Caroline A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/089603383X
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