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03889nam a22003135i 4500 |
| 001 |
978-1-59259-566-2 |
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20191027061606.0 |
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cr nn 008mamaa |
| 008 |
100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595662
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| 024 |
7 |
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|a 10.1385/0896033856
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Plant Virology Protocols
|b From Virus Isolation to Transgenic Resistance /
|c edited by Gary D. Foster, Sally Taylor.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XV, 571 p. 111 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 81
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| 505 |
0 |
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|a to Plant Virology -- to Classical Crossprotection -- History of Coat Protein-Mediated Protection -- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes -- Geminivirus Isolation and DNA Extraction -- Caulimovirus Isolation and DNA Extraction -- Reovirus Isolation and RNA Extraction -- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction -- Hordeivirus Isolation and RNA Extraction -- Furovirus Isolation and RNA Extraction -- Tobravirus Isolation and RNA Extraction -- Tobamovirus Isolation and RNA Extraction -- Potexvirus Isolation and RNA Extraction -- Carlavirus Isolation and RNA Extraction -- Potyvirus Isolation and RNA Extraction -- Trichovirus Isolation and RNA Extraction -- Ilarvirus Isolation and RNA Extraction -- Bromovirus Isolation and RNA Extraction -- Cucumovirus Isolation and RNA Extraction -- Nepovirus Isolation and RNA Extraction -- Comovirus Isolation and RNA Extraction -- Carmovirus Isolation and RNA Extraction -- Tymovirus Isolation and Genomic RNA Extraction -- Tombusvirus Isolation and RNA Extraction -- Luteovirus Isolation and RNA Extraction -- Cloning of Coat Protein Genes -- RNA Analysis -- RNA Fractionation by Density Gradient Centrifugation -- cDNA Library Construction for the Lambda ZAP®-Based Vectors -- PCR Cloning of Coat Protein Genes -- Antibody Production -- Expression Library Screening -- In Vitro Transcription and Translation -- Analysis of Coat Protein Expression Cassettes in Protoplasts -- DNA Sequencing -- Computer Analysis of Amino Acid Sequences -- Plant Transformation and Primary Analysis -- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation -- Potato Transformation -- Transformation of Tomato -- Tobacco Transformation -- Genetic Transformation of Wheat -- Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309) -- Molecular Analysis of Transgenic Rice -- PCR Analysis of Transgenic Tobacco Plants -- Southern Analysis of Transgenic Tobacco Plants -- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes -- Western Analysis of Transgenic Plants -- Evaluation of Resistance -- Assaying Levels of Plant Virus by ELISA -- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization -- Detection and Quantification of Plant Viruses by PCR -- Assaying Levels of Virus with Local Lesion Hosts -- Field Testing Resistance of Transgenic Plants -- Agronomic Performance of Transgenic Plants -- Mechanisms of Resistance -- Mechanisms of Resistance -- Mechanisms of RNA-Mediated Resistance to Plant Viruses -- Detection of Risks Associated with Coat Protein Transgenics -- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.
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| 650 |
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|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Foster, Gary D.
|e editor.
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| 700 |
1 |
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|a Taylor, Sally.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033856
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