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02787nam a22002895i 4500 |
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978-1-59259-567-9 |
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20190617231745.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
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|a 9781592595679
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| 024 |
7 |
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|a 10.1385/089603495X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Receptor Signal Transduction Protocols
|c edited by R. A. J. Challiss.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a XIII, 275 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 83
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| 505 |
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|a Methods for Characterizing Receptors -- Radioligand-Binding Methods for Membrane Preparations and Intact Cells -- Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding -- Approaches to the Stable Transfection of G Protein-Coupled Receptors -- Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels -- The Generation of Receptor-Selective Antibodies -- In Situ Hybridization -- lmmunocytochemical Methods for Investigating Receptor Localization -- Methods for Studying Receptor Coupling Mechanisms -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes -- Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding -- Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes -- Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling -- Identification and Quantitation of G Protein ?-Subunits -- Covalent Modification of G Proteins by Affinity Labeling -- Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection -- Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C -- Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons -- Methods for Investigating the Regulation of Receptor Function -- Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation -- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation -- Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells -- Regulation of Receptor Expression.
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| 650 |
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|a Neurosciences.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 700 |
1 |
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|a Challiss, R. A. J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603495X
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