Receptor Signal Transduction Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
|
| Edición: | 1st ed. 1997. |
| Colección: | Methods in Molecular Biology,
83 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/089603495X |
Tabla de Contenidos:
- Methods for Characterizing Receptors
- Radioligand-Binding Methods for Membrane Preparations and Intact Cells
- Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding
- Approaches to the Stable Transfection of G Protein-Coupled Receptors
- Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels
- The Generation of Receptor-Selective Antibodies
- In Situ Hybridization
- lmmunocytochemical Methods for Investigating Receptor Localization
- Methods for Studying Receptor Coupling Mechanisms
- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes
- Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding
- Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes
- Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling
- Identification and Quantitation of G Protein ?-Subunits
- Covalent Modification of G Proteins by Affinity Labeling
- Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection
- Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C
- Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons
- Methods for Investigating the Regulation of Receptor Function
- Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation
- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation
- Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells
- Regulation of Receptor Expression.