Bioluminescence Methods and Protocols
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
102 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896035204 |
Tabla de Contenidos:
- The Basics
- Improvements in the Application of Firefly Luciferase Assays
- Visualization of Bioluminescence
- Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone
- Analytical Biochemistry
- Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue
- Bioluminescent Assay of the Guanylates
- Bioluminescent Assay of the Adenylate Energy Charge
- Molecular Biology Tools
- Photorhabdus luminescens luxCDABE Promoter Probe Vectors
- Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome
- Probing for Promoters with Luciferase-Transposons
- Cryopreservation and Reawakening
- Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists
- Cell-Based Assays
- Genotoxic Sensors
- Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response
- Real-Time Reporter of Protein Synthesis Inhibition
- Luminescence-Based Cell Viability Testing
- Phagocyte Chemiluminescence
- Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters
- Environmental Applications
- Luciferase-Based Measurement of Water Contaminants
- Bioluminescence-Based Metal Detectors
- Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters
- A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity
- Organic Contaminant Detection and Biodegradation Characteristics
- Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples
- Monitoring of GFP-Tagged Bacterial Cells.