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01837nam a22003015i 4500 |
| 001 |
978-1-59259-578-5 |
| 005 |
20191027071353.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595785
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| 024 |
7 |
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|a 10.1385/0896034216
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Pichia Protocols
|c edited by David R. Higgins, James Cregg.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XI, 270 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 103
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| 505 |
0 |
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|a to Pichia pastoris -- Classical Genetic Manipulation -- Transformation -- Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection -- The Generation of Multicopy Recombinant Strains -- Isolation of Nucleic Acids -- Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression -- Glycosylation Profiling of Heterologous Proteins -- High Cell-Density Fermentation -- Use of Pichia pastoris as a Model Eukaryotic System -- Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I) -- Secretion of scFv Antibody Fragments -- Expression of Tetanus Toxin Fragment C -- Expression of EGF and HIV Envelope Glycoprotein -- Expression of an Integral Membrane Protein, the 5HT5A Receptor -- Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Higgins, David R.
|e editor.
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| 700 |
1 |
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|a Cregg, James.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896034216
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