Pichia Protocols
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edition: | 1st ed. 1998. |
| Series: | Methods in Molecular Biology,
103 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896034216 |
Table of Contents:
- to Pichia pastoris
- Classical Genetic Manipulation
- Transformation
- Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection
- The Generation of Multicopy Recombinant Strains
- Isolation of Nucleic Acids
- Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression
- Glycosylation Profiling of Heterologous Proteins
- High Cell-Density Fermentation
- Use of Pichia pastoris as a Model Eukaryotic System
- Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
- Secretion of scFv Antibody Fragments
- Expression of Tetanus Toxin Fragment C
- Expression of EGF and HIV Envelope Glycoprotein
- Expression of an Integral Membrane Protein, the 5HT5A Receptor
- Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections.