Pichia Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
|
| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
103 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896034216 |
Tabla de Contenidos:
- to Pichia pastoris
- Classical Genetic Manipulation
- Transformation
- Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection
- The Generation of Multicopy Recombinant Strains
- Isolation of Nucleic Acids
- Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression
- Glycosylation Profiling of Heterologous Proteins
- High Cell-Density Fermentation
- Use of Pichia pastoris as a Model Eukaryotic System
- Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
- Secretion of scFv Antibody Fragments
- Expression of Tetanus Toxin Fragment C
- Expression of EGF and HIV Envelope Glycoprotein
- Expression of an Integral Membrane Protein, the 5HT5A Receptor
- Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections.