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02544nam a22003015i 4500 |
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978-1-59259-594-5 |
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20190618121647.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
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|a 9781592595945
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| 024 |
7 |
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|a 10.1385/0896033473
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Herpes Simplex Virus Protocols
|c edited by S. Moira Brown, Alasdair R. MacLean.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XI, 419 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 10
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| 505 |
0 |
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|a HSV Growth, Preparation, and Assay -- HSV Entry and Spread -- Preparation of HSV-DNA and Production of Infectious Virus -- HSV Mutagenesis -- Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions -- Construction and Use of Cell Lines Expressing HSV Genes -- Analysis of HSV Polypeptides -- Protein Purification -- Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells -- Crystallization of Macromolecules for Three-Dimensional Structure Determination -- Direct Immunogold Labeling of Herpesvirus Suspensions -- Expression of HSV Proteins in Bacteria -- In Vitro Systems to Analyze HSV Transcript Processing -- Analysis of HSV-1 Transcripts by RNA-PCR -- Transient Assays for HSV Origin and Replication Protein Function -- HSV Amplicons in Gene Therapy -- Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions -- HSV-Cellular Protein Interactions -- Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse -- Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit -- HSV Latency In Vitro -- Assessing Cell-Mediated Immune Responses to HSV in Murine Systems -- Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection -- Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction -- HSV Vectors for Gene Therapy -- Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Brown, S. Moira.
|e editor.
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| 700 |
1 |
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|a MacLean, Alasdair R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033473
|