Neurodegeneration Methods and Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Harry, Jean. (Editor), Tilson, Hugh A. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edition:1st ed. 1999.
Series:Methods in Molecular Medicine, 22
Subjects:
Online Access:https://doi.org/10.1385/089603612X
Table of Contents:
  • to Polymerase Chain Reaction
  • Methodological Issues in Polymerase Chain Reaction for RNA
  • Subtractive Hybridization of cDNA Libraries
  • The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in Ischemic Brain Injury
  • Analysis of Gene Expression by Multiprobe RNase Protection Assay
  • Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration
  • Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis
  • Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage
  • Detection of Apoptotic Cells in the Nervous System
  • Translocation Assays of Protein Kinase C Activation
  • Astrocyte and Neuron Coculturing Method
  • Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the Immunofluorescence Combination Assay
  • Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay
  • Measurement of Intraneuronal Free Calcium Using the Fluorescent Probe Technique
  • Measurement of Calcium Buffering by Intracellular Organelles in Brain
  • Receptor-Mediated Release of Inositolphosphates in Brain Slices
  • The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins in Biological Functions
  • Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders
  • Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue
  • The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD
  • Biochemical Measurement of Cholinesterase Activity
  • Functional Study of Glutamate Receptor Channels in Brain Slices
  • Selective Silver Staining for Neural Degeneration
  • Immunohistological Markers of Cerebrovascular Integrity
  • Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells.