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02686nam a22002895i 4500 |
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978-1-59259-655-3 |
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20190618121756.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592596553
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|a 10.1385/159259655X
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Purification Protocols
|c edited by Paul Cutler.
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| 250 |
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|a 2nd ed. 2004.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XII, 484 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 244
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|a General Strategies -- Preparation of Extracts From Animal Tissues -- Protein Extraction From Plant Tissues -- Extraction of Recombinant Protein From Bacteria -- Protein Extraction From Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal-Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Thiophilic Affinity Chromatography and Related Methods -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent From Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins From Polyacrylamide Gels -- Electroblotting of Proteins From Polyacrylamide Gels -- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses -- Microscale Solution Isoelectrofocusing -- Practical Column Chromatography -- Detection Methods -- Peptide Proteomics -- Multidimensional Liquid Chromatography of Proteins -- Mass Spectrometry -- Purification of Therapeutic Proteins -- Purification Process Scale-Up.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Cutler, Paul.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/159259655X
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