Protein Purification Protocols
| Corporate Author: | |
|---|---|
| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
|
| Edition: | 2nd ed. 2004. |
| Series: | Methods in Molecular Biology,
244 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/159259655X |
Table of Contents:
- General Strategies
- Preparation of Extracts From Animal Tissues
- Protein Extraction From Plant Tissues
- Extraction of Recombinant Protein From Bacteria
- Protein Extraction From Fungi
- Subcellular Fractionation of Animal Tissues
- Subcellular Fractionation of Plant Tissues
- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds
- Avoidance of Proteolysis in Extracts
- Concentration of Extracts
- Making and Changing Buffers
- Purification and Concentration by Ultrafiltration
- Bulk Purification by Fractional precipitation
- Ion-Exchange Chromatography
- Hydrophobic Interaction Chromatography
- Affinity Chromatography
- Dye-Ligand Affinity Chromatography
- Lectin Affinity Chromatography
- Immunoaffinity Chromatography
- Immobilized Metal-Ion Affinity Chromatography
- Chromatography on Hydroxyapatite
- Thiophilic Affinity Chromatography and Related Methods
- Affinity Precipitation Methods
- Isoelectric Focusing
- Chromatofocusing
- Size-Exclusion Chromatography
- Fast Protein Liquid Chromatography
- Reversed-Phase Chromatography of Proteins
- Extraction of Membrane Proteins
- Removal of Detergent From Protein Fractions
- Purification of Membrane Proteins
- Lyophilization of Proteins
- Storage of Pure Proteins
- Electroelution of Proteins From Polyacrylamide Gels
- Electroblotting of Proteins From Polyacrylamide Gels
- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses
- Microscale Solution Isoelectrofocusing
- Practical Column Chromatography
- Detection Methods
- Peptide Proteomics
- Multidimensional Liquid Chromatography of Proteins
- Mass Spectrometry
- Purification of Therapeutic Proteins
- Purification Process Scale-Up.