Molecular Diagnosis of Infectious Diseases

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Decker, Jochen. (Editor), Reischl, Udo. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edition:2nd ed. 2004.
Series:Methods in Molecular Medicine, 94
Subjects:
Online Access:https://doi.org/10.1385/1592596797
Table of Contents:
  • Protein-Based Identification Of Antigens
  • Proteomic Approaches to Antigen Discovery
  • Immunoproteomics
  • Immunoprecipitation and Blotting
  • DNA-Based Identification of Antigens
  • Representational Difference Analysis of cDNA
  • Microarray Data Analysis and Mining
  • Expression Cloning
  • Identification of Epitopes and Immunomodulatory Components
  • Determination of Epitopes by Mass Spectrometry
  • Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays
  • Virus-like Particles
  • Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
  • Expression of Recombinant Proteins
  • High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
  • Production of Antigens in Chlamydomonas reinhardtii
  • Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
  • Purification, Modification, and Renaturation of Recombinant Proteins
  • Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
  • Purification of Recombinant Proteins with High Isoelectric Points
  • Refolding of Inclusion Body Proteins
  • Small-Molecule-Protein Conjugation Procedures
  • Characterization of Recombinant Proteins
  • Structural Characterization of Proteins and Peptides
  • Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
  • Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
  • Evaluation of Recombinant Proteins in Immunological Test Systems
  • Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
  • Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
  • Basic Problems of Serological Laboratory Diagnosis
  • Molecular Diagnostics Resources on the Internet
  • Recombinant Receptor Molecules
  • Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.