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02463nam a22002895i 4500 |
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978-1-59259-686-7 |
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20190618021454.0 |
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cr nn 008mamaa |
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100301s2000 xxu| s |||| 0|eng d |
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|a 9781592596867
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|a 10.1385/159259686X
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Transcription Factor Protocols
|c edited by Martin J. Tymms.
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|a 1st ed. 2000.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2000.
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| 300 |
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|a X, 306 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 130
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|a Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method -- In Vivo Footprinting Using UV Light and Ligation-Mediated PCR -- Identification of DNaseI Hypersensitive Sites Within Nuclei -- Analysis of In Vivo Methylation -- Detection of Transcription Factor Partners with a Yeast One Hybrid Screen -- Inverse PCR (IPCR) for Obtaining Promoter Sequence -- PCR-Directed Linker Scanning Mutagenesis -- Transfection Technologies -- The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues -- Optimizing Electroporation Conditions for the Transformation of Mammalian Cells -- Calcium Phosphate Transfection of Mammalian Cultured Cells -- DEAE-Dextran Transfection of Mammalian Cultured Cells -- Liposome-Mediated Transfection of Mammalian Cells -- Assays for Transcriptional Activity Based on the Luciferase Reporter Gene -- Transient Transfection of Schneider Cells in the Study of Transcription Factors -- Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription -- Expression and Purification of Histidine-Tagged Transcription Factors -- Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein -- Electrophoretic Mobility Shift Assays -- In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors -- In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors -- Computer Software of Eukaryotic Promoter Analysis.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Human Genetics.
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| 700 |
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|a Tymms, Martin J.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/159259686X
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